And by mulligan, I mean can I go back about 3 months in time?
I have been struggling to find a good way to purify my protein out of cells in order to study it further. I will spare you the details, but for a number of reasons this has been very difficult. And finally, after months of frustration, I found (or so I thought) a way to do it that didn't destroy the function of my protein. Go me.
And then this morning as I sat doing a bit of troubleshooting I had one of those giant light bulb moments. My strategy wasn't going to work - and for the most incredibly obvious reason - one that I should have thought of about 3 months ago. All the work that I was going to do this week and most of the work that I've done for the past 3 months......all for nothing. To say that I was unhappy would be an understatement. I wasn't angry, but very sad - disappointed with my skills. Why did it take me this long to have this revelation?
I had to tell my boss, there was no way to escape that. Happily, he took it very well and that helped a bit.
What makes this worse was that I had already planned on having an "I'm being kind of slow and I need your help" moment with my boss today. I find it's ok to have these moments, and in fact the PI's kind of like them since they feel needed, as long as you spread them out a bit.
Basically, I need to find a reagent in a published manuscript. My boss told me what reagent and told me the senior author's name and said "Go, fetch." I tried, I really did. I searched through all the manuscripts that came from the particular lab that I could find. There was no mention of this anywhere, but my boss was convinced, so I was convinced that I just wasn't looking the right way. Finally, I realized I really did need some help and had planned on talking to the boss about it today. And then stupid moment number one happened and I decided to postpone talking to my boss about stupid thing number 2 until tomorrow.
Sadly, he didn't get the memo and managed to ask me about that on his own. Ugh. In the end though, I was redeemed - at least for this. Turns out that what he had sent me searching for wasn't actually in any published manuscript. He was remembering a conversation in passing with one particular PI....clearly I wasn't going to be finding that on-line. As soon as this dawned on him, my boss said that he would go e-mail the other PI and see what they could come up with and he'd get back to me. (Insert sigh of relief)
In the end, I don't feel quite as ridiculous as I did this morning. I was only a little slow about one thing, not two and I've already taken the first steps to correcting that mistake. Really, these things do happen and all you can do is (to borrow one of my dad's favorite quotes, from the movie "Heartbreak Ridge.") "improvise, adapt and overcome."